FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24091296
Sequences flagged as poor quality0
Sequence length75
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA31750.030.14596450
GCGTCGT34050.029.67528212
GCCGTAT33600.028.9813348
GATCGGA40850.025.311952
CGCCGTA41600.024.28980647
AGCGTCG48200.021.3786311
ATCGGAA49400.021.0659873
CCGTATC47400.020.82471749
AGAGCGT50600.020.3645829
TGGTCGC47400.019.84042443
GTCGCCG49500.019.13293345
AGATCGG55150.018.5670741
TCGCCGT49650.018.40370246
TCGTGTA61650.017.9525215
CGTGTAG62150.017.75482216
CGTCGTG59500.017.65425313
GTCGTGT63400.017.35258114
GTAGATC58500.017.10084332
GGTCGCC57900.016.58724444
TCTCGGT65100.016.13631237
Produced by FastQC (version 0.11.5)