FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859123

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859123
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31833875
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG36250.011.97217914
CGAAGAT107700.011.345711
TTACACG36600.011.19060832
CCGGGAT54050.010.9474481
CGCGGGT16800.010.4203581
CGCAGAC70850.010.2788711
CGTTCGC22300.010.20159316
TCGCGCG5552.6237103E-810.0891318
CGACCGT11750.09.8332711
CGATCCA45150.09.7665144
TCGGGTC59800.09.7157742
CGCGGTT18900.09.6330421
CGCGTTC23950.09.49877114
CTCGGGT77150.09.4848891
CGGGGAT58000.09.4171291
CGCTCGT46000.09.20634215
CGCCAAC58950.09.1465821
CTCGGAT74200.09.1069991
ATAGCCG36400.09.03805621
CCGGCAT51500.08.9740531
Produced by FastQC (version 0.11.5)