FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48606058
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG406400.029.85552443
CTCGTAT418300.028.65162842
ACACGTC445500.028.47404913
CACGTCT472550.026.85128614
ACGTCTG472300.026.80706215
TCTCGTA449700.026.68940541
GATCGGA414300.025.758981
TATGCCG471850.025.49470546
CGTATGC476450.025.4153344
ATCTCGT476700.025.27905340
CACACGT503000.025.26021612
AATCTCG468900.025.10360739
ACCAATC487500.023.97572136
GCCGTCT495200.023.8537449
GACCAAT501350.023.7880635
GCACACG549000.023.57728811
CGTCTGA540550.023.5564216
ATCGGAA454900.023.4097542
AGATCGG287750.023.0822981
ATGCCGT531000.022.4793447
Produced by FastQC (version 0.11.5)