FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25636904
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTCG16200.021.7169833
TCGTATG35200.021.2759145
TATGCCG41100.018.3910848
CGTATGC41100.018.05446446
TATCGCG11250.016.24915920
CTCGTAT47650.016.22206544
ACACGTC51250.016.21981813
ACGTCTG54150.015.8601615
GATCGGA48500.014.7457511
CGTACGT8500.013.79677834
AGTCACG46900.013.60548528
CACGTCT65450.013.22744214
TCTCGTA59000.012.8614843
CGACCTA14900.012.500684
ATCGGAA58600.012.2628462
CGAAGAT80000.012.0058551
GTCACGT53200.011.92954529
ATCTCGT65450.011.91022342
TAAGTCG27250.011.77128820
AATCTCG37500.011.68136441
Produced by FastQC (version 0.11.5)