FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29613662
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT46050.027.66404542
ACACGTC54250.024.35205313
TATGCCG54900.023.08232146
GATCGGA53600.022.1104011
CGTATGC63400.019.98755544
TCGTATG67550.018.759643
ATGCCGT69450.018.73030547
TCTCGTA66550.018.58685341
CACGTCT71000.018.5596814
ACTTAGG71450.018.49283432
ACGTCTG77650.017.4031315
CACTTAG77900.016.8756831
TTAGGCA86800.015.95882634
CTTAGGC89650.015.3389833
ATCTCGT81450.015.06287540
ATCGGAA79100.015.004812
CACACGT89250.014.98974112
TCACTTA92150.014.65611930
TCGGAAG84100.014.3889873
GCACACG106250.014.20374911
Produced by FastQC (version 0.11.5)