FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44716873
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTAT57350.011.1530581
CCCGTAT45950.09.8125181
CCGGGAT91300.09.7238711
CGTATCG38250.09.5024973
TTACGCG27650.09.45841754
CCGGAAT95000.09.3819451
CCCGATA93100.09.31389769
GTATCGA43600.09.2977694
ACTCGTG97800.09.18265922
ACCCGAT98050.09.15959768
TCGCTCG95700.09.12059414
CGTATCA38950.09.10023550
TACTCGT100550.08.86125221
CGCAGAC135900.08.7445151
CGCGTAA34400.08.6364791
TACGCGA31500.08.52085355
CGCTCGT104200.08.50915215
TAACGCC46000.08.45322753
ACTTCGC112400.08.44044611
CCGGCAT85950.08.4178411
Produced by FastQC (version 0.11.5)