FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37142091
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCGA39800.014.5433084
TTACGCG27950.014.25322354
TACGCGA32500.012.46737455
GCGGTAT57950.012.0660611
CGTATCG45700.011.9206833
CCCGTAT43800.011.91468051
ATCGAAG81400.011.7956866
CGCGTAT6251.8189894E-1110.9147821
CCGACAT68650.010.4338311
ACGCGAC40800.010.01457356
GTTACGC43400.09.64990953
CGCGGAT33650.09.2240451
TCGAAGG115600.09.1895727
CCGATAA49150.09.14434120
CCGGCAT76400.09.0182671
CCGCAAT39800.08.9985251
CGAAGAT99850.08.9499031
CATACGA17150.08.9340815
CCGGGAT89000.08.8912541
GTCGCTA43350.08.71828827
Produced by FastQC (version 0.11.5)