FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15400792
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG37500.023.65931143
TATGCCG45350.019.56386846
CGTATGC46100.019.31966644
CTCGTAT42150.019.26842
GATCGGA51600.018.7334371
ACACGTC54450.017.54735813
ATCGGAA55650.017.32142
TCTCGTA47500.016.73834441
ACGTCTG61500.015.9796615
TCACGAT55350.015.41228135
CACGATC51850.015.39967736
ACGATCT50600.015.37548637
CACGTCT65750.014.73920814
ATCACGA63550.014.65857134
ATGCCGT63600.014.37915947
ATCTCGT55850.014.29693240
TCGGAAG71250.013.7505243
TCGCTCG21750.013.49244814
CATCACG73250.013.18327533
CACACGT73100.013.16386212
Produced by FastQC (version 0.11.5)