FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41183907
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG132100.032.3676221
TCGTATG144300.031.53659446
CGTATGC151600.030.01837347
TATGCCG151750.029.87545849
GTTCCGT139050.028.33309236
CGTATCT138300.028.2623140
ACACGTC171250.027.67889214
TATCTCG142250.027.5749342
CTCGTAT163700.027.54445645
GATCGGA156450.027.4555452
CCGTATC151200.026.01075639
TCCGTAT150250.025.9226538
TTCCGTA155950.025.32905637
ACGTCTG192900.025.00158116
TCTCGTA185250.024.1726244
CACGTCT197300.024.0593515
CACACGT207750.022.9488613
ATCTCGT197250.022.85937143
ATCGGAA187900.022.8234183
ATGCCGT202450.022.5300250