FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41183907
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA96800.046.6730550
GCGTCGT105300.043.44865412
GCCGTAT104700.043.19669348
GATCGGA117800.039.8117642
CGCCGTA117900.038.44930647
AGCGTCG129550.035.6086711
CCGTATC129300.035.05759449
AGAGCGT133050.034.931949
AGATCGG137150.033.8161051
GTAGATC137550.032.99909232
TGGTCGC138450.032.3214643
CGTCGTG146200.031.74320413
ATCGGAA150550.031.0799563
TCGCCGT145000.030.93042246
CGTGTAG152700.030.7293916
GTCGTGT153400.030.25462514
GTATCAT153650.029.78304151
GGTCGCC153500.029.77968644
TCGTGTA156900.029.6650215
AAGAGCG160250.029.261388
Produced by FastQC (version 0.11.5)