FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20592064
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG24750.037.18887743
CGTATGC27400.033.0179244
TATGCCG27450.032.3852446
CTCGTAT34500.025.91921242
ACACGTC37350.025.84612513
GATCGGA37300.024.5154111
CACGTCT49400.019.8843914
TCTCGTA47150.018.67792541
ATGCCGT50900.018.3150447
ACGTCTG55050.017.78200115
ATCGGAA53600.017.1063882
TATCGCG20900.016.6926322
TCGGAAG57500.016.259683
CACACGT61550.015.51875912
ATCTCGT56450.015.24076540
GCCGTCT63250.014.49014549
GCACACG74150.014.20643711
CCCGTAT20600.013.7760681
CGCGTAA11400.013.1382861
CATCTCG69150.013.12726839
Produced by FastQC (version 0.11.5)