FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20592064
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG19550.016.9464122
TTACACG29550.014.59167432
CCCGTAT22900.014.3145941
CGTATCG20750.013.9695943
GTATCGA19750.011.7065294
CGACCTA8900.011.6319284
GCGGTAT39150.011.1934211
TTACGCG15100.011.18834754
CGCTAAT12650.010.90376434
CTAGTAC21950.010.8445229
ATTCCGT40400.010.66968153
ATCGAAG41050.010.5923586
ATATCGC31950.010.47723521
ATCGCGG31700.010.45193923
ACACGAA43550.010.29347934
GTCGCTA14800.010.26072227
ACGGACA42550.010.13412618
CGAAGAT70600.010.1171121
TACGCGA16650.09.93898855
CGCGATT11150.09.89831417
Produced by FastQC (version 0.11.5)