FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32765116
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG28850.010.64250843
CGAAGAT97250.010.5171391
CCGGGAT65200.010.2813391
TTACGCG5402.1427695E-810.221642554
CGCGTAT5301.7414641E-79.7793711
CGTATGC36050.09.28252644
TATGCCG35750.09.16746646
CTCGGTT43200.08.9583871
CTCGGAT78150.08.84293651
CTCGAAT55050.08.5992151
CGCGAAT12100.08.5670521
CGCGAAA12100.08.55333843
CTCGTAT40000.08.5383742
CGGGGAT71650.08.5359641
TCGCTCG41700.08.43857714
TATCGCG7001.2783858E-78.3784820
CGCCAAT54350.08.3285091
CCGGCAT63500.08.2167251
CCGGAAT60750.08.0767671
CGCGGAT29250.08.0330151
Produced by FastQC (version 0.11.5)