FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22098584
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG4852.3646862E-1112.80558322
CGACCTA8500.011.7702264
CGAAGAT69350.011.1615931
CCGGGAT43500.010.7243051
CTCGGAT55100.09.8462911
CGCGATT7809.094947E-129.7465911
CTCGGTT25050.09.6563931
TCGCTCG28900.09.4304214
CGGGGAT44350.09.3500141
CGCGAAT10200.09.1472011
CTCGTTT31800.09.1280251
CGCAAAT23850.09.1280251
TAAGCGA15200.08.85168146
CCGGAAT39450.08.847061
CGCCAAT41200.08.8067711
TTACACG23900.08.80500832
CTCGAAT38500.08.5268281
TAAGTCG17500.08.47709320
TCGGATC28900.08.47557452
CGACGAT20050.08.4451291
Produced by FastQC (version 0.11.5)