FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22098584
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATT7250.012.3849871
TCGCTCG30700.011.91255514
CGAAGAT67700.011.4266671
CCGGGAT44050.011.0543561
TAAGCGA15000.011.03846746
CTCGGTT25000.010.2223781
CTCAGAT128900.010.0738431
CGCCAAT41800.09.8317521
CTCGGAT56350.09.5607261
CCGGCAT43000.09.5573771
CTCGGGT54000.09.5291191
CGCGGAT19400.09.2568211
CGACCTA10450.09.2450584
CGCAGAC68550.09.1186681
CGGGGAT46650.09.1057081
CCGGAAT36700.09.0336941
CGGGATT23350.09.0220071
CGCCAAC43050.08.9847321
TCGGGTC42850.08.9379932
CGCAGAT39900.08.8285121
Produced by FastQC (version 0.11.5)