FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32104542
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA34950.017.62517450
GCCGTAT39150.015.46615248
CGCCGTA41600.014.63941847
GCGTCGT47650.013.88242912
AGCGTCG55900.011.896296511
GTCGCCG49950.011.84183845
GATCGGA58550.011.8362742
CCGTATC56700.011.04937949
TCGGGTC58300.010.6262862
CTCGGGT75650.010.1786731
TAAGTCG29250.010.17090620
ATCGGAA69700.09.7922073
AGAGCGT70000.09.7000449
TGGTCGC60650.09.46410743
CGCGTTC24100.09.29457414
CCGGGAT68950.09.289541
CGAAGAT105900.09.2211621
CGTCGTG78000.09.19868713
CTCGGAT77650.09.1502291
AGATCGG76950.09.0515271
Produced by FastQC (version 0.11.5)