FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34999105
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG28550.014.76612554
TACGCGA32900.012.70953355
GCGGTAT48800.012.6411881
CGTATCA32050.012.191051550
GCGTCGT34650.011.12788412
GTTACGC40000.010.88217653
ACGCGAC39050.010.79370256
GCCGTAT40700.09.68545948
ATCGAAG67350.09.68285756
GATCGGA45850.09.6194312
CCGGCAT61500.09.5058671
CGTTCGC25500.09.47644216
TAACGCC44500.09.31960253
GTATCGA31500.09.2227784
CGAACGA50450.09.10384327
TAGCGCG31250.08.97926318
CCGATAA51100.08.92278120
CGCCGTA44200.08.91902347
CCCGTAT35550.08.777261
GCGATCG48650.08.73681334
Produced by FastQC (version 0.11.5)