FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27836628
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA35400.016.15993750
TCGCTCG36950.015.20741714
GATCGGA66300.015.1784281
GCCGTAT41150.013.73395448
GCGTCGT46100.013.0947312
GTCGCCG45000.013.0194445
TAAGCGA20050.012.89066946
ATCGGAA78500.012.6438782
CGCTCGT45350.012.39577515
CGCCGTA49100.012.28225547
CGCGAAC6250.012.1310981
AGCGTCG58800.011.67263210
CGCAGAC75850.011.13186551
AGAGCGT67200.010.8792448
CGTATCG22350.010.7939083
ACTCGTG54600.010.48074822
TGGTCGC52150.010.44573343
TCGCCGT57800.010.43371146
CCGTATC56250.010.41500949
TCGGAAG95400.010.2956723
Produced by FastQC (version 0.11.5)