FastQCFastQC Report
Sun 5 May 2024
EGAF00004859157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40872996
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT26217086.414279002204781TruSeq Adapter, Index 15 (97% over 40bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTA13997603.4246571990954613TruSeq Adapter, Index 15 (97% over 40bp)
AGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCT1216240.2975656592435749TruSeq Adapter, Index 15 (96% over 33bp)
AAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTC747190.18280773936904454TruSeq Adapter, Index 15 (97% over 34bp)
AGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTC694790.16998753896093158TruSeq Adapter, Index 15 (96% over 31bp)
CACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTG653830.15996625253504784TruSeq Adapter, Index 15 (96% over 29bp)
ACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGC539540.1320040253471999TruSeq Adapter, Index 15 (96% over 28bp)
GAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGT516480.12636215852637767TruSeq Adapter, Index 15 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATTTCGTAT483740.11835197987443836TruSeq Adapter, Index 15 (97% over 40bp)
ACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTT424480.10385340971824039RNA PCR Primer, Index 40 (96% over 29bp)

[OK]Adapter Content

Adapter graph