FastQCFastQC Report
Fri 3 May 2024
EGAF00004859158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40872996
Total Bases3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC8431462.0628436437593174Illumina Single End PCR Primer 1 (100% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG4987591.220265331173668Illumina Single End PCR Primer 1 (100% over 50bp)
CAAGCAGAAGACGGCATACGAGATTCTGACATGTGACTGGAGTTCAGACG1927320.4715387147054256RNA PCR Primer, Index 40 (96% over 29bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTAGATCTCGGTGGTCGCC671120.16419642934909887Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGGGGTCGCCG523680.12812371278092752Illumina Single End PCR Primer 1 (98% over 50bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGGGTAGATCTCGGTGGTCGCCG461770.11297679279492992Illumina Single End PCR Primer 1 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)