FastQCFastQC Report
Wed 27 Apr 2022
EGAF00004859158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40872996
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAA974700.23847040720968923Illumina Single End PCR Primer 1 (100% over 58bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAAAAA611570.1496269077020926Illumina Single End PCR Primer 1 (100% over 57bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAAAAAAAAAGAA573720.14036651485004914Illumina Single End PCR Primer 1 (100% over 58bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)