FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28112124
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG63950.031.66829546
CTCGTAT62900.031.53716942
ACACGTC68850.030.27044713
CGTATGC73500.027.78826944
GATCGGA70050.027.246811
TCGTATG77450.026.41427843
CACGTCT83350.025.29422814
TCTCGTA76950.025.28575941
ACTTAGG88000.023.95045732
ATGCCGT86950.023.0930947
ACGTCTG91500.022.99768415
CACTTAG93000.022.77386931
CACACGT95150.021.94119312
TTAGGCA101050.020.99413334
CTTAGGC104950.020.148133
TCGGAAG96550.020.1170333
TCACTTA109100.019.34956230
ATCTCGT99500.019.2073540
GCCGTCT104500.019.14779549
ATCGGAA102400.018.6308632
Produced by FastQC (version 0.11.5)