FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47079579
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA889170.18886532524005792TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA660130.1402157823034059TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG335800.048.2098271
TCGTATG511700.031.55473746
TATGCCG512450.030.92373849
CGTATGC516000.030.91784547
GATCGGA526550.030.7837092
ACACGTC576100.030.36016314
CTCGTAT526100.030.2170845
TCTCGTA546050.029.28367844
ACGTCTG604900.029.137116
CACGTCT602850.028.99583815
ATCGGAA563550.028.909533
TCGGAAG596350.028.7975484
CACACGT624150.028.13346713
ATGCCGT553650.028.0180650
ATCTCGT578350.027.9402843
CGTCTGA632000.027.89856717
AATCTCG575950.027.76933942
GCACACG651800.027.23129312
GCCGTCT581900.026.47381252
GTATGCC610750.026.40415848
Produced by FastQC (version 0.11.5)