FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47079579
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA284950.058.62689650
CGCCGTA292650.057.43063747
GCCGTAT301400.055.77354448
GCGTCGT306250.055.2656312
CCGTATC320100.052.3739849
GATCGGA339100.051.5258032
TGGTCGC331400.050.21996743
GTCGTGT332150.050.2103814
AGATCGG344850.050.0138361
AGCGTCG343850.049.93482611
TCGTGTA334200.049.87784615
AGAGCGT346000.049.7053389
TCGCCGT333100.049.5355846
CGTGTAG340100.049.45860316
CGTCGTG344850.049.29094713
GGTCGCC343150.049.1804344
GTAGATC344300.048.69499232
TCTCGGT359400.047.4020337
GTATCAT356500.046.98291451
ATCGGAA372750.046.963793
Produced by FastQC (version 0.11.5)