FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36349964
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGC25050.010.47893316
CGCGTAT7603.6379788E-1210.1328261
CGTATCG26450.09.9242823
CGCGAAA14650.09.79521443
CGACCGT17450.09.4280931
CGCGTTC27900.09.40850414
CTCGTTT57500.09.3750671
CTCGGGT85150.09.2495231
GTATCGA36600.09.0846054
CCGGGAT83200.09.0035111
CGCGGAT28400.08.9975851
CTCGGAT84700.08.9267191
TAAGTCG34050.08.83987420
CGCGAGT20200.08.8377211
TCGCTCG52750.08.75820314
CCCGTAT39600.08.6626821
CGCCAAT72700.08.5705181
TCGGGTC61300.08.5645252
CTCGGTT49900.08.5581671
CCGCGTT32350.08.43886113
Produced by FastQC (version 0.11.5)