FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32158178
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA24100.016.0320471
CGTATCG25650.015.31905653
CCCGTAT30300.013.532271
TTACGCG16700.012.98055254
TCGTATG19400.012.7774943
GTATCGA28650.012.768594
TCGTTCG37500.011.4735928
CCGTATC40900.011.4536472
TACGCGA19650.011.20446955
TCGGGTC59200.010.9054172
GCGTAAC36150.010.7807032
TTACACG34300.010.47006732
CGGGGTT71200.010.4655421
GCGGTAT45550.010.3000311
CGACCTA15800.010.2902994
ATCGAAG60250.010.2308156
GTTCGTT81200.010.13864330
TTATCGG42700.010.0744935
CGGTATA18550.09.9741092
GCCGTAT21650.09.83367351
Produced by FastQC (version 0.11.5)