FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32158178
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG29000.017.0163443
CCCGTAT32850.015.7564521
CGCGTAA25450.014.9144551
GTATCGA31050.014.6703064
CCGTATC48800.012.7285872
TTACACG34700.012.22724832
ATCGAAG60450.011.1893336
CGAGCGA57200.010.73127753
TCGTTCG37000.010.44685928
GCCGTAT27300.010.3646281
GCGTAAC38600.010.2810642
TTACGCG18200.010.23002854
GCGGTAT43900.010.0611571
TATCGAA42600.09.9641435
CGCGTAT4855.555685E-79.9606771
CGCGAAT10750.09.9507481
CCCGTCG59950.09.900151
CGATCCA45500.09.7837464
CTCGGTT40700.09.750031
GTTCGTT83900.09.70495930
Produced by FastQC (version 0.11.5)