FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56870036
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG149100.026.90809246
AGATCGG155700.025.5110261
CGTATGC162700.024.65877347
TATGCCG162200.024.60717849
CTCGTAT169450.023.6765845
TCCGTAT165400.023.20542738
GTTCCGT167950.023.16136736
TATCTCG161750.023.01229142
CGTATCT165000.022.8012940
ACACGTC198750.022.70288513
GATCGGA180850.021.9602322
CCGTATC183750.021.0568639
TCTCGTA205900.019.61923843
TTCCGTA201300.019.4784737
CACGTCT233250.019.37448114
ACGTCTG241100.018.92962316
ATGCCGT222150.018.01328550
CACACGT253750.017.945212
CGTCTGA265450.017.07622716
TCGGAAG255950.016.9451054
Produced by FastQC (version 0.11.5)