FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25840355
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG24000.017.11462222
CGACCTA13650.014.6544914
TTACACG37850.013.21631932
ATATCGC32100.012.90270721
GCGGTAT40750.012.1016881
TTACGCG10500.011.83013754
CGGTATA19250.010.9289352
CGCTAAT13350.010.85825420
ATCGCGG38150.010.85719123
CTAACGG13650.010.11611469
ACTAACG12600.09.862617568
CGACTTA21700.09.86150767
GCGGTGT45000.09.8092311
GCCGTAT28500.09.8011651
ACACGAA56000.09.79208834
TTGCGTA18000.09.77760366
GCGGGTT28750.09.7159371
CGCGTAA12950.09.5867241
ATTGCGT17650.09.38284265
TACACGA58900.09.36898833
Produced by FastQC (version 0.11.5)