FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20647289
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG102450.042.00303343
TATGCCG104100.041.66438346
CGTATGC104550.041.4204844
CTCGTAT105450.039.5459642
GATCGGA120550.037.99251
ACACGTC123100.037.09752713
ATGCCGT121300.035.81275647
ATCGGAA129000.035.608432
ACGTCTG128800.035.4014215
AATCTCG119350.034.5398139
TCTCGTA118350.034.4281441
CACGTCT135650.033.61503214
GCCGTCT131850.033.128249
CCGTCTT135950.032.35502650
TGCCGTC137750.031.88269248
ATCTCGT127950.031.81819540
TCGGAAG146550.031.5533643
CACACGT146800.031.38734812
CGTCTGA147450.031.0822116
GCACACG152400.030.45796211
Produced by FastQC (version 0.11.5)