FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53793587
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG134950.043.74018545
TATGCCG141950.041.34018348
CGTATGC142950.041.21992546
CTCGTAT151850.037.39542844
ACACGTC169050.037.0196613
GATCGGA147300.034.8496481
ACGTCTG198600.031.4935815
CACGTCT203100.031.00002914
TCTCGTA187700.030.4370143
ATGCCGT194400.030.06219349
CGTCTGA219700.028.6730716
CACACGT225350.028.04650112
ATCTCGT210900.027.02336142
ATCGGAA197450.025.9140422
GCCGTCT232750.025.0792351
TCGGAAG209600.024.938533
GCACACG267750.024.2880511
GTATGCC250150.024.10706947
AATCTCG211100.023.7784741
TGCCGTC257650.022.76262350
Produced by FastQC (version 0.11.5)