FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53793587
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG28600.014.40155354
CGTATCA49500.013.30442450
CGCCGTA50450.013.12451647
TACGCGA32650.012.40491455
GCGTCGT58650.011.42060812
GCCGTAT60600.010.75489648
GCGGTAT56800.010.4146261
ATCGAAC60850.09.8318286
ACGCGAC43100.09.63612956
GATCGGA72300.09.5564632
CCGGAAT97000.09.4346791
GTTACGC48250.09.17237853
GCGCGAT37450.09.1057841
AATCGAA76750.09.066025
TACTCGT102400.09.01082421
CCCGATA100800.08.94752669
CCGGGAT108850.08.855121
CGCAGAC158550.08.7129931
ACTCGTG108100.08.69803422
TCGCTCG106900.08.65297614
Produced by FastQC (version 0.11.5)