FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24016795
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG26200.023.43803243
TATGCCG30150.020.25413546
CGTATGC31050.019.6670644
ACACGTC38250.016.68865813
AGTCACG38250.016.41300628
CTCGTAT36900.016.2676742
TATCTCG37800.015.97057939
CGTATCG22400.015.094023
TTACGCG15500.014.69131954
GATCGGA39900.014.532871
GCGGTAT40100.014.2882381
CGCGTAA20800.013.9389781
GTCACGC48450.012.95763629
CACGTCT53000.012.63010514
TACGCGA18500.012.49544255
CGCCAAT56350.012.36558633
GTATCGA27350.012.3622724
CCCGTAT28050.012.3050151
CCGTATC31450.011.9572892
ATCGAAG50200.011.5465526
Produced by FastQC (version 0.11.5)