FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24016795
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG23800.017.8841693
GTATCGA24000.016.6000844
CGTATCA20600.014.71158350
TTACGCG14300.013.81136754
CGACCTA12350.012.9588544
CCCGTAT29550.012.8136581
GCGGTAT38650.012.7975281
ATCGAAG47850.012.52467256
GCGTCGT26650.012.26631812
TACGCGA16800.012.16151255
GCCGTAT25950.011.67855848
CGATCCA36250.011.3661424
CCGTATC41950.011.12052
TTACACG25000.010.62427532
ACGCGAC19750.010.51742256
GATCGGA29900.010.4773712
CCGCAAT24200.010.4309521
TATCGAA33150.010.3746525
GCGCGAT18750.010.18812751
TAACGCC24000.010.07355629
Produced by FastQC (version 0.11.5)