FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26608595
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35400.028.01305843
CGTATGC37550.026.59124444
TATGCCG42300.023.44372746
CTCGTAT46300.021.78722442
TATCTCG45850.021.62811739
TATCGCG14550.020.68057422
AGTCACG49900.020.35735128
GATCGGA49400.020.1016331
ACACGTC57450.018.03587213
CGCCAAT65300.015.71573333
CACGTCT69200.015.3687614
GTCACGC70800.014.63933229
TCTCGTA68000.014.38195741
ATGCCGT70250.014.16494447
CACACGT75500.013.99574712
ATCTCGT71100.013.8029940
ACGTCTG76650.013.78576415
ATCGGAA73400.013.6059622
TCGGAAG80550.012.9907643
CGTCTGA87200.012.23527316
Produced by FastQC (version 0.11.5)