FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16124925
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG13150.019.15032443
TATCGCG7450.016.67130722
TATGCCG15750.016.64608246
CGTATGC15550.016.4164944
TTACGCG22100.014.67289954
CTCGTAT18050.014.33385742
TACGCGA23600.013.88647355
GTTACGA12050.013.7459024
TGTTACG12500.013.5303033
GCGGTAT39500.012.6072111
ATCGAAG34700.012.1283746
GTTACGC27550.012.02059453
GATCGGA23000.010.8257571
CCAATCG18150.010.64344715
CGTGCGA28250.010.62396131
ACGCGAC30850.010.62300656
CCGACAT30950.010.6149171
GCGATCG29200.010.39626834
GTCGCTA21300.010.04157527
ACACGTC26650.09.96699813
Produced by FastQC (version 0.11.5)