FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29819866
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG38500.014.60724514
CGCTCGT46600.012.21625415
CGCAGAC67800.011.499321
CGAAGAT93450.010.4841311
TCGTGCG56450.010.3865524
ACTCGTG58400.010.21691122
CTCGGTT33450.010.2101591
TCGGGTC48700.09.9172282
CTCGGAT64250.09.6647981
CGGGGAT53150.09.6062051
CGTGCGC60350.09.60112225
TACTCGT60750.09.59479121
CGGACAT50350.09.5922821
CGTTCGC15900.09.5476316
CGATCCA42750.09.3607874
CGCCTCG64150.09.30398729
GCTCGTA65000.09.28890916
CTCGGGT62800.09.17382051
CTCGTAC62950.09.15294417
CGACAAT41950.09.1281851
Produced by FastQC (version 0.11.5)