FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26338505
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA71450.033.197361
TATGCCG70550.032.8934946
TCGTATG70900.032.6754643
CGTATGC73950.031.56265844
ACACGTC80250.031.3850313
CTCGTAT79400.028.359842
ATCGGAA84650.028.0189862
ACGTCTG90150.027.6715915
TCTCGTA85750.025.82131241
CACGTCT98600.025.6467214
ATGCCGT92100.025.08587347
ATCTCGT96500.023.15729740
TCGGAAG109250.021.9591223
CGTCTGA113500.021.88685616
CACACGT112250.021.18112
GCACACG117650.020.88159811
GCCGTCT112700.020.53252649
AATCTCG60350.019.81655739
TGCCGTC118450.019.68008448
CCGTCTT122250.019.23603650
Produced by FastQC (version 0.11.5)