FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28065259
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA92900.028.7446671
ACACGTC114050.027.52625813
ACGTCTG122350.025.79752315
ATCGGAA104750.025.5235922
CACGTCT127700.024.58171314
GTCACGA45350.022.74328629
TCGGAAG131000.020.7777023
CACACGT153300.020.63613112
AGTCACG52500.020.63144528
CGTCTGA156400.020.29128616
GCACACG168750.019.42138111
CGCGTAA4500.018.4005491
AGATCGG76100.017.7718851
ACGATCA57850.017.65037232
CGGAAGA181350.017.4236354
CACGATC59850.017.00291331
TTACACG36450.016.65638232
TCGTATG93550.016.29833443
TCTGAAC203500.016.1881618
GTCTGAA201350.015.8641917
Produced by FastQC (version 0.11.5)