FastQCFastQC Report
Tue 7 May 2024
EGAF00004859205

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859205
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17936970
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC1709850.9532546466878185TruSeq Adapter, Index 9 (100% over 50bp)
CGTATGCCGTCTTCTGCTTGAGATCGGAAGAGCACACGTCTGAACTCCAG276710.15426797279585125Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG207180.11550445811081805TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)