FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45681684
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG66650.029.55641643
TATGCCG72300.027.19857646
CGTATGC75050.026.15636444
CTCGTAT97400.021.5357742
GATCGGA83800.020.5874771
ACACGTC105350.019.0258713
TCTCGTA106750.018.29223341
CACGTCT121750.016.77484714
ACGTCTG126200.016.1013315
ATGCCGT127450.015.53734847
ATCTCGT128550.015.45854440
CACACGT142850.014.15223812
TCGGAAG140750.012.7227743
GCCGTCT157900.012.65085449
CGTCTGA162550.012.606812516
GCACACG174850.012.37113711
ATCGGAA146150.011.8040682
GATCTCG149500.011.76920639
GTATGCC173050.011.62285345
TGCCGTC177600.011.48028648
Produced by FastQC (version 0.11.5)