FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45681684
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGCG30800.012.95987254
TACGCGA36400.011.15510455
CGTATCG39150.010.802253
CCCGTAT45850.010.5963051
CGTATCA38750.010.56810850
CCGGGAT100900.010.53081
GTATCGA43300.010.4926844
GCGGTAT56300.010.2436451
GCGTCGT49500.09.47832312
ACGCGAC44250.09.17618656
GCATACG31750.09.12739214
CATACGA24600.09.11589715
TAGCGCG36100.08.89513718
CGCCGTA50800.08.7407947
GTTACGC47150.08.68480753
ATCGAAG91250.08.53694256
CGCGTAA46350.08.5213351
CCGGAAT107100.08.4852141
CCGGCAT88200.08.4409241
GCCGTAT50050.08.390017548
Produced by FastQC (version 0.11.5)