FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41307683
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCG63800.013.60502114
CGCTCGT74650.011.53381615
CGAAGAT138650.011.1574881
CGCAGAC119000.010.6763731
TATATCG24300.09.93825235
CGCGAAA18450.09.86458643
CTCGGGT100750.09.8311751
ACTCGTG89500.09.69824622
TACTCGT90250.09.57887121
TAAGCGA28000.09.50008546
TCGGGTC79200.09.4569842
CTCGGAT97750.09.4525981
CCGGCAT77350.09.4116771
CTCGTAC93850.09.28608817
CCCGATA93150.09.2431569
CCGGGAT93650.09.2310941
TCGTGCG95050.09.02149624
CGTTCGC30700.09.00650716
CGACAAT57550.08.8791541
AACCCGA103700.08.87648667
Produced by FastQC (version 0.11.5)