FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26971208
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG35100.032.25065643
TATGCCG35350.030.61944446
CGTATGC38400.028.95281244
TATCTCG44250.026.26710539
GATCGGA44250.025.6798651
CTCGTAT49750.024.17579342
ACACGTC52100.022.70146613
ACTAGCT60700.020.09203132
TAGCTTA61450.019.84684634
CACTAGC61800.019.40731631
TCTCGTA62950.018.57117541
CACGTCT66050.018.314914
ATGCCGT63650.018.11050647
TATCGCG14600.017.76816722
TCACTAG70000.016.8931530
ACGTCTG71300.016.82139615
ATCGGAA69300.016.6280442
GCTTATC72900.016.49853936
ATCTCGT72550.015.60300640
TCGGAAG75550.015.5324533
Produced by FastQC (version 0.11.5)