FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31415733
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46200.014.69685745
TATGCCG47300.014.5762948
CGCGTTC21500.013.18514
CGCGGGT17150.012.8584161
CGTATGC55350.012.3931946
CGACCTA17750.012.2238594
CGCGAGT12750.012.0796281
CGTTCGC21450.012.07367316
CTCGTAT58800.011.66826744
CCGCGTT23250.011.59033113
TATCTCG60900.011.20859541
CGCGAAA12650.010.7921543
CCGGGAT64600.010.7828021
ACACGTC67000.010.49903413
GATCGGA60150.010.3570322
CGCGCAT10150.010.3458521
CTCGGGT68850.09.9646761
TTACACG36350.09.91976232
TCGGGTC56500.09.8491692
CGCGGAT23600.09.7891051
Produced by FastQC (version 0.11.5)