FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23496405
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG48950.031.2212143
CGTATGC53200.028.8567344
TATGCCG52700.028.80289846
TATCTCG57950.027.02685739
CTCGTAT58600.025.78547942
TAGCTTA67150.024.45350834
ACACGTC68800.024.32644713
GATCGGA62500.023.6816061
ACTAGCT72000.022.51819432
CACTAGC72800.022.46028131
CACGTCT78850.021.39996714
GCTTATC77700.020.5561236
TCTCGTA74700.020.32033341
ACGTCTG87500.019.39691515
TCACTAG84950.019.16659730
ATGCCGT79300.018.88041547
ATCTCGT82250.018.6645440
CACACGT90500.018.64635512
AGATCGG50750.017.9474071
CGTCTGA98450.017.41467516
Produced by FastQC (version 0.11.5)