FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48599210
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA731120.1504386593938461TruSeq Adapter, Index 7 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAA528340.10871370131325181TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG306500.030.05460448
TCGTATG308600.029.98424145
CGTATGC313850.029.4277146
GTCACGA318100.029.06815329
TATCTCG314750.029.02293841
AGATCGG259500.028.1798781
ACACGTC336600.028.0200313
AGTCACG332900.027.85875928
CACGAGT319550.027.74857131
ACGAGTG322800.027.53332532
CTCGTAT335200.027.53192744
ACGTCTG357700.026.52151915
TCTCGTA349000.026.36250743
ATGCCGT347150.026.33655449
TCACGAG358350.026.0920530
CACGTCT369550.025.58706714
GCCGTCT323400.025.37815751
GCCGTAT67700.025.37636252
GATCGGA382100.024.7972981
ATCTCGT373050.024.69994442
Produced by FastQC (version 0.11.5)