FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48599210
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA199650.033.81387347
CGTATCA196250.033.7141650
GCCGTAT207450.032.72540748
GTCGCCG198250.032.36451745
TCGCCGT207350.029.8463946
CCGTATC229200.029.42426149
TGGTCGC209550.029.07183543
GGTCGCC230750.027.85098544
AGATCGG193250.027.123951
GCGTCGT191600.026.32561112
TCTCGGT260250.025.57483137
CGGTGGT245450.025.18933740
GTAGATC271750.024.86022432
TCGGTGG272500.024.52476939
GGCGCCG86200.024.4113644
GTATCAT274150.024.3984951
GTGGTCG263750.023.33683442
CTCGGTG291850.022.90020838
AGCGTCG220500.022.82786211
CGTCGTG228100.022.37020113
Produced by FastQC (version 0.11.5)