FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34977346
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG46850.029.82077443
CGTATGC50150.028.0647744
TATGCCG50550.027.91093446
CTCGTAT58300.024.49639542
TATCTCG62450.021.98472239
GATCGGA65300.021.9700361
ACACGTC69650.020.95389613
CACCGAT69850.020.7970131
ACCGATG70750.020.77630832
CGTATCG34650.018.2180583
CCCGTAT37500.017.7918821
CGATGTA82550.017.47277334
CCGATGT83050.017.11828233
CACGTCT87350.016.94493514
ATGCCGT90000.015.90661647
TCTCGTA89900.015.8858741
GTATCGA37750.015.8078274
ATCTCGT91400.015.62516240
CACACGT101800.014.40329412
CCGTATC52300.014.3144822
Produced by FastQC (version 0.11.5)