FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004859220

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004859220
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34977346
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCG32000.016.8216673
CCCGTAT33100.016.2626381
GTATCGA31850.016.1425154
CGTATCA31400.014.72453350
CGCGTAA26600.014.6585571
GCGTCGT38050.013.32367312
GCCGTAT36200.012.96258948
GCGGTAT50600.012.4794371
ATCGAAG69600.011.5026536
CGAGCGA56050.010.9515453
TTACGCG20000.010.86011754
CGCGTAT5754.5292836E-1010.8018731
CCGTATC58250.010.7812692
GATCGGA46800.010.7646872
CGCCGTA44200.010.53835847
GCGTAAC38300.010.3608052
TCGTTCG38700.010.34455128
CGATCCA46050.010.1157614
CGCGCGT32350.09.9198041
TATCGAA53350.09.7675
Produced by FastQC (version 0.11.5)